May 11, 2021

Download Ebook Free Advanced Methods In Molecular Biology And Biotechnology

Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Release Date : 2020-11-10
Category : Science
Total pages :218
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Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology
Author : John M. Walker,Ralph Rapley,E. B. Gingold
Publisher : Royal Society of Chemistry
Release Date : 2000
Category : Science
Total pages :563
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This volume provides a revised and updated introduction to the techniques of molecular biology and its industrial applications. It should be of particular benefit to undergraduates and researchers in other biological areas.

Methods in Plant Molecular Biology and Biotechnology

Methods in Plant Molecular Biology and Biotechnology
Author : Bernard R. Glick
Publisher : CRC Press
Release Date : 2018-05-04
Category : Science
Total pages :382
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Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology
Author : S.L. Salzberg,D.B. Searls,S. Kasif
Publisher : Elsevier
Release Date : 1998-06-19
Category : Computers
Total pages :368
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Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Molecular Biology Techniques

Molecular Biology Techniques
Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Release Date : 2019-03-05
Category : Science
Total pages :294
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Advanced Molecular Genetics

Advanced Molecular Genetics
Author : Alfred Pühler,Kenneth N. Timmis
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
Category : Science
Total pages :350
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The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.

Advanced Biotechnology

Advanced Biotechnology
Author : R C Dubey
Publisher : S. Chand Publishing
Release Date : 2014
Category : Science
Total pages :1161
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The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Departments of Labor, Health and Human Services, Education, and Related Agencies Appropriations for 1992

Departments of Labor, Health and Human Services, Education, and Related Agencies Appropriations for 1992
Author : United States. Congress. House. Committee on Appropriations. Subcommittee on the Departments of Labor, Health and Human Services, Education, and Related Agencies
Publisher : Unknown
Release Date : 1991
Category : United States
Total pages :129
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Molecular Biology Techniques

Molecular Biology Techniques
Author : Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publisher : Academic Press
Release Date : 2012
Category : Medical
Total pages :200
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release Date : 2010-03-04
Category : Medical
Total pages :744
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Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
Author : Rajan Katoch
Publisher : Springer Science & Business Media
Release Date : 2011-07-19
Category : Science
Total pages :441
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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology
Author : Robert A. Meyers
Publisher : John Wiley & Sons
Release Date : 1995-06-29
Category : Medical
Total pages :1034
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Essential for the top of every desk and laboratory bench, this single volume reference is the first and last resource required to answer any question relating to molecular biology, biotechnology, or molecular medicine. This work contains the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. Over 250 carefully reviewed articles written by 378 eminent scientists in the field, provide detailed and readable coverage of today's most relevant topics including life processes at the molecular level, genetic disease diagnosis and genetic therapy, the theory and techniques for understanding, manipulating, and synthesizing biological molecules and their aggregates.

The Molecular and Cellular Biology of the Potato

The Molecular and Cellular Biology of the Potato
Author : Michael E. Vayda,William R. Belknap,William D. Park
Publisher : C A B International
Release Date : 1994
Category : Science
Total pages :270
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Potato improvement by traditional breeding and opprotunities for new technologies; Ac-Ds trasposons mapped near disease resistance loci for targeted tagging in potato; Studies to enhance starch biosynthesis by manipulation of ADP-glucose pyrophsphorylase genes; Control of carbohydrate metabolism in potato tubers; Transgenic potatoes changed in carbohidrate partitioning and allocation; Control of sugar balance in potato tubers; Post-harvest regulation of sucrose accumulation in transgenic potatoes: role and properties of potato tuver UDP-glucose pyrophosphorylase; Post-harvest potato tuver glycerolipid; Functionality of semi-artificial trasnit peptides encoded by gene constructs derived form hte potato gene for granule-bound starch synthase; Potato alternative oxidase: detection of mRNA by PCR and tissue-specific differences in the protein levels; Polyphenol oxidase in potato tubers; Gene expression during early tuber development; Use of ubiquitin promoters for transgene; Regulation of translation in potato tubers in response to environmental stress; Expression of the Brasil nut methuibube-rich protein in transgenic potato plants; Strategies towards introducing resistance to bacterial pathogens in transgenic potatoes; Field performance of transgenic potatoes; Analysis of containment and food safety issues associated with the release of transgenic potatoes.

Biotechnology and Integrated Pest Management

Biotechnology and Integrated Pest Management
Author : C.A.B. International
Publisher : Biotechnology in Agriculture
Release Date : 1996
Category : Science
Total pages :475
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This text presents a spectrum of views on the benefits and risks in the use of biotechnology in integrated pest management. It assesses the likelihood of new technologies being usefully incorporated into IPM programmes, and discusses types of new biotechnologies.

Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology
Author : Frank H. Stephenson
Publisher : Academic Press
Release Date : 2016-06-16
Category : Computers
Total pages :496
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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts