April 13, 2021

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Bioconjugate Techniques

Bioconjugate Techniques
Author : Greg T. Hermanson
Publisher : Academic Press
Release Date : 2013-07-25
Category : Science
Total pages :1200
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Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Bioconjugation Protocols

Bioconjugation Protocols
Author : Sonny S. Mark
Publisher : Humana Press
Release Date : 2016-08-23
Category : Science
Total pages :605
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Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Drug-like Properties: Concepts, Structure Design and Methods

Drug-like Properties: Concepts, Structure Design and Methods
Author : Li Di,Edward H Kerns
Publisher : Elsevier
Release Date : 2010-07-26
Category : Science
Total pages :552
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Of the thousands of novel compounds that a drug discovery project team invents and that bind to the therapeutic target, typically only a fraction of these have sufficient ADME/Tox properties to become a drug product. Understanding ADME/Tox is critical for all drug researchers, owing to its increasing importance in advancing high quality candidates to clinical studies and the processes of drug discovery. If the properties are weak, the candidate will have a high risk of failure or be less desirable as a drug product. This book is a tool and resource for scientists engaged in, or preparing for, the selection and optimization process. The authors describe how properties affect in vivo pharmacological activity and impact in vitro assays. Individual drug-like properties are discussed from a practical point of view, such as solubility, permeability and metabolic stability, with regard to fundamental understanding, applications of property data in drug discovery and examples of structural modifications that have achieved improved property performance. The authors also review various methods for the screening (high throughput), diagnosis (medium throughput) and in-depth (low throughput) analysis of drug properties. * Serves as an essential working handbook aimed at scientists and students in medicinal chemistry * Provides practical, step-by-step guidance on property fundamentals, effects, structure-property relationships, and structure modification strategies * Discusses improvements in pharmacokinetics from a practical chemist's standpoint

Bioconjugation

Bioconjugation
Author : Sam Massa,Nick Devoogdt
Publisher : Humana
Release Date : 2019-07-23
Category : Medical
Total pages :317
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This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.

Immobilized Affinity Ligand Techniques

Immobilized Affinity Ligand Techniques
Author : Greg T. Hermanson,A. Krishna Mallia,Paul K. Smith
Publisher : Academic Press
Release Date : 1992-09-10
Category : Science
Total pages :454
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This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References

Enhanced Cytosolic Delivery of DNA by a Sulfhydryl-activatable Listeriolysin O Bioconjugate

Enhanced Cytosolic Delivery of DNA by a Sulfhydryl-activatable Listeriolysin O Bioconjugate
Author : Go Saito
Publisher : Unknown
Release Date : 2002
Category :
Total pages :129
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Radiopharmaceutical Chemistry

Radiopharmaceutical Chemistry
Author : Jason S. Lewis,Albert D. Windhorst,Brian M. Zeglis
Publisher : Springer
Release Date : 2019-04-02
Category : Medical
Total pages :651
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This book is a comprehensive guide to radiopharmaceutical chemistry. The stunning clinical successes of nuclear imaging and targeted radiotherapy have resulted in rapid growth in the field of radiopharmaceutical chemistry, an essential component of nuclear medicine and radiology. However, at this point, interest in the field outpaces the academic and educational infrastructure needed to train radiopharmaceutical chemists. For example, the vast majority of texts that address radiopharmaceutical chemistry do so only peripherally, focusing instead on nuclear chemistry (i.e. nuclear reactions in reactors), heavy element radiochemistry (i.e. the decomposition of radioactive waste), or solely on the clinical applications of radiopharmaceuticals (e.g. the use of PET tracers in oncology). This text fills that gap by focusing on the chemistry of radiopharmaceuticals, with key coverage of how that knowledge translates to the development of diagnostic and therapeutic radiopharmaceuticals for the clinic. The text is divided into three overarching sections: First Principles, Radiochemistry, and Special Topics. The first is a general overview covering fundamental and broad issues like “The Production of Radionuclides” and “Basics of Radiochemistry”. The second section is the main focus of the book. In this section, each chapter’s author will delve much deeper into the subject matter, covering both well established and state-of-the-art techniques in radiopharmaceutical chemistry. This section will be divided according to radionuclide and will include chapters on radiolabeling methods using all of the common nuclides employed in radiopharmaceuticals, including four chapters on the ubiquitously used fluorine-18 and a “Best of the Rest” chapter to cover emerging radionuclides. Finally, the third section of the book is dedicated to special topics with important information for radiochemists, including “Bioconjugation Methods,” “Click Chemistry in Radiochemistry”, and “Radiochemical Instrumentation.” This is an ideal educational guide for nuclear medicine physicians, radiologists, and radiopharmaceutical chemists, as well as residents and trainees in all of these areas.

Chemical Reagents for Protein Modification, Fourth Edition

Chemical Reagents for Protein Modification, Fourth Edition
Author : Roger L. Lundblad
Publisher : CRC Press
Release Date : 2014-07-22
Category : Medical
Total pages :684
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The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.

RNA Methodologies

RNA Methodologies
Author : Robert E. Farrell, Jr.
Publisher : Elsevier
Release Date : 2010-07-22
Category : Science
Total pages :688
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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Chemistry of Bioconjugates

Chemistry of Bioconjugates
Author : Ravin Narain
Publisher : John Wiley & Sons
Release Date : 2013-12-02
Category : Science
Total pages :496
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Explores bioconjugate properties and applications ofpolymers, dendrimers, lipids, nanoparticles, and nanotubes Bioconjugation has enabled breakthroughs across many areas ofindustry and biomedicine. With its emphasis on synthesis,properties and applications, this book enables readers tounderstand the connection between chemistry and the biologicalapplication of bioconjugated materials. Its detailed descriptionsof methods make it possible for researchers to fabricate and takefull advantage of bioconjugates for a broad range of applications.Moreover, the book sets the foundation for the development of newapplications, including assays, imaging, biosensors, drug delivery,and diagnostics. Chemistry of Bioconjugates features contributions from aninternational team of leading experts and pioneers in the field.These contributions reflect the authors’ firsthand laboratoryexperience as well as a thorough review of the current literature.The book’s six sections examine: General methods of bioconjugation Polymer bioconjugates Organic nanoparticle-based bioconjugates Inorganic nanomaterial bioconjugates, including metals andmetal oxides Cell-based, hydrogel/microgel, and glyco-bioconjugates Characterization, physico-(bio)chemical properties, andapplications of bioconjugates This comprehensive exploration of bioconjugates includesdiscussions of polymers, dendrimers, lipids, nanoparticles, andnanotubes. References at the end of each chapter serve as a gatewayto the most important original research findings and reviews in thefield. By drawing together and analyzing all the latest chemicalmethods and research findings on the physico-chemical andbiochemical properties of bioconjugates, Chemistry ofBioconjugates sheds new light on the significance and potentialof bioconjugation. The book is recommended for organic and polymerchemists, biochemists, biomaterial scientists, carbohydratechemists, biophysicists, bioengineers, and drug and gene deliveryscientists.

Chemoselective and Bioorthogonal Ligation Reactions

Chemoselective and Bioorthogonal Ligation Reactions
Author : W. Russ Algar,Philip Dawson,Igor L. Medintz
Publisher : John Wiley & Sons
Release Date : 2017-03-17
Category : Technology & Engineering
Total pages :430
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This timely, one-stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others.

Gold Nanostars

Gold Nanostars
Author : Giuseppe Chirico,Mykola Borzenkov,Piersandro Pallavicini
Publisher : Springer
Release Date : 2015-08-03
Category : Technology & Engineering
Total pages :80
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This Brief focuses on the synthesis, functionalization techniques, optical properties and biomedical application of gold nanostars (GNS). Various facilities of gold nanostars synthesis as well as functionalization of GNS with PEG, organic dyes, bioactive compounds are discussed. The authors discuss physical origin of the Localized Surface Plasmon Resonances and the way the nano-environment affects them. The implication of the LSPR of gold nanostars surface enhanced Raman scattering is also discussed. The emphasis has been done on the application of GNS for current and emerge needs of medicine, biology and pharmacy. Moreover, properties of gold nanostars as contrast agents for in vivo imaging and interaction of GNS with cells are also discussed in this Brief.

Encyclopedia of Molecular Biology, Volume 2

Encyclopedia of Molecular Biology, Volume 2
Author : Thomas E. Creighton
Publisher : Wiley-Interscience
Release Date : 1999
Category : Science
Total pages :2856
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Annotation The field of molecular biology has revolutionized the study of biology. The applications to medicine are enormous, ranging from diagnostic techniques for disease and genetic disorders, to drugs, to gene therapy. Focusing on the fundamentals of molecular biology and encompassing all aspects of the expression of genetic information, the Encyclopedia of Molecular Biology will become the first point of reference for both newcomers and established professionals in molecular biology needing to learn about any particular aspect of the field.

Handbook of Fluorescent Probes and Research Products

Handbook of Fluorescent Probes and Research Products
Author : Richard P. Haugland
Publisher : Unknown
Release Date : 2002
Category : Fluorescent probes
Total pages :966
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Bio-inspired Polymers

Bio-inspired Polymers
Author : Nico Bruns,Andreas F M Kilbinger
Publisher : Royal Society of Chemistry
Release Date : 2016-10-14
Category : Science
Total pages :612
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Many key aspects of life are based on naturally occurring polymers, such as polysaccharides, proteins and DNA. Unsurprisingly, their molecular functionalities, macromolecular structures and material properties are providing inspiration for designing new polymeric materials with specific functions, for example, responsive, adaptive and self-healing materials. Bio-inspired Polymers covers all aspects of the subject, ranging from the synthesis of novel polymers, to structure-property relationships, materials with advanced properties and applications of bio-inspired polymers in such diverse fields as drug delivery, tissue engineering, optical materials and lightweight structural materials. Written and edited by leading experts on the topic, the book provides a comprehensive review and essential graduate level text on bio-inspired polymers for biochemists, materials scientists and chemists working in both industry and academia.