November 27, 2020

Download Ebook Free Bioconjugate Techniques

Bioconjugate Techniques

Bioconjugate Techniques
Author : Greg T. Hermanson
Publisher : Academic Press
Release Date : 2013-07-25
Category : Science
Total pages :1200
GET BOOK

Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Bioconjugate Techniques

Bioconjugate Techniques
Author : Greg T. Hermanson
Publisher : Academic Press
Release Date : 2010-07-26
Category : Science
Total pages :1323
GET BOOK

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs,fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions

Drug-like Properties: Concepts, Structure Design and Methods

Drug-like Properties: Concepts, Structure Design and Methods
Author : Li Di,Edward H Kerns
Publisher : Elsevier
Release Date : 2010-07-26
Category : Science
Total pages :552
GET BOOK

Of the thousands of novel compounds that a drug discovery project team invents and that bind to the therapeutic target, typically only a fraction of these have sufficient ADME/Tox properties to become a drug product. Understanding ADME/Tox is critical for all drug researchers, owing to its increasing importance in advancing high quality candidates to clinical studies and the processes of drug discovery. If the properties are weak, the candidate will have a high risk of failure or be less desirable as a drug product. This book is a tool and resource for scientists engaged in, or preparing for, the selection and optimization process. The authors describe how properties affect in vivo pharmacological activity and impact in vitro assays. Individual drug-like properties are discussed from a practical point of view, such as solubility, permeability and metabolic stability, with regard to fundamental understanding, applications of property data in drug discovery and examples of structural modifications that have achieved improved property performance. The authors also review various methods for the screening (high throughput), diagnosis (medium throughput) and in-depth (low throughput) analysis of drug properties. * Serves as an essential working handbook aimed at scientists and students in medicinal chemistry * Provides practical, step-by-step guidance on property fundamentals, effects, structure-property relationships, and structure modification strategies * Discusses improvements in pharmacokinetics from a practical chemist's standpoint

Bioconjugation Protocols

Bioconjugation Protocols
Author : Sonny S. Mark
Publisher : Humana Press
Release Date : 2016-08-23
Category : Science
Total pages :605
GET BOOK

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Chemistry of Bioconjugates

Chemistry of Bioconjugates
Author : Ravin Narain
Publisher : John Wiley & Sons
Release Date : 2013-12-02
Category : Science
Total pages :496
GET BOOK

Explores bioconjugate properties and applications ofpolymers, dendrimers, lipids, nanoparticles, and nanotubes Bioconjugation has enabled breakthroughs across many areas ofindustry and biomedicine. With its emphasis on synthesis,properties and applications, this book enables readers tounderstand the connection between chemistry and the biologicalapplication of bioconjugated materials. Its detailed descriptionsof methods make it possible for researchers to fabricate and takefull advantage of bioconjugates for a broad range of applications.Moreover, the book sets the foundation for the development of newapplications, including assays, imaging, biosensors, drug delivery,and diagnostics. Chemistry of Bioconjugates features contributions from aninternational team of leading experts and pioneers in the field.These contributions reflect the authors’ firsthand laboratoryexperience as well as a thorough review of the current literature.The book’s six sections examine: General methods of bioconjugation Polymer bioconjugates Organic nanoparticle-based bioconjugates Inorganic nanomaterial bioconjugates, including metals andmetal oxides Cell-based, hydrogel/microgel, and glyco-bioconjugates Characterization, physico-(bio)chemical properties, andapplications of bioconjugates This comprehensive exploration of bioconjugates includesdiscussions of polymers, dendrimers, lipids, nanoparticles, andnanotubes. References at the end of each chapter serve as a gatewayto the most important original research findings and reviews in thefield. By drawing together and analyzing all the latest chemicalmethods and research findings on the physico-chemical andbiochemical properties of bioconjugates, Chemistry ofBioconjugates sheds new light on the significance and potentialof bioconjugation. The book is recommended for organic and polymerchemists, biochemists, biomaterial scientists, carbohydratechemists, biophysicists, bioengineers, and drug and gene deliveryscientists.

Bioconjugation

Bioconjugation
Author : Sam Massa,Nick Devoogdt
Publisher : Humana
Release Date : 2019-07-23
Category : Medical
Total pages :317
GET BOOK

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.

Immobilized Affinity Ligand Techniques

Immobilized Affinity Ligand Techniques
Author : Greg T. Hermanson,A. Krishna Mallia,Paul K. Smith
Publisher : Academic Press
Release Date : 1992-09-10
Category : Science
Total pages :454
GET BOOK

This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References

Chemical Reagents for Protein Modification, Fourth Edition

Chemical Reagents for Protein Modification, Fourth Edition
Author : Roger L. Lundblad
Publisher : CRC Press
Release Date : 2014-07-22
Category : Medical
Total pages :684
GET BOOK

The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.

15-Minute Counseling Techniques That Work

15-Minute Counseling Techniques That Work
Author : Allison Edwards
Publisher : 15-Minute Focus
Release Date : 2020-05-21
Category : Education
Total pages :80
GET BOOK

The counselor is not the strategy. The counselor teaches strategies. As counselors, we spend our days helping kids. Kids come to us with a variety of problems, searching for answers. They want us to listen. And they need us to give them solutions for the issues they are facing. While these solutions may work temporarily, we really never help kids until we give them tools"¬‚¬"or techniques"¬‚¬"to manage thoughts and feelings on their own. Our job is not to do it for them. Our job is to teach them how to do it themselves! This is the greatest gift we can give. In 15-Minute Counseling Techniques, Allison Edwards provides tools to use in individual or group counseling sessions with children in grades K"¬‚¬"12. Children will learn how to calm their mind and body with Square Breathing, let go of negative thoughts by Changing the Channel, identify their unique gifts by creating a "What I'm Good At" Jar, and so much more. The techniques in this book will help children feel empowered to face everyday challenges and equipped to manage their stress and emotions. And, best of all, you will give them the confidence they need to handle challenges throughout their lives.

Engineered Carbohydrate-Based Materials for Biomedical Applications

Engineered Carbohydrate-Based Materials for Biomedical Applications
Author : Ravin Narain
Publisher : John Wiley & Sons
Release Date : 2011-03-01
Category : Technology & Engineering
Total pages :416
GET BOOK

This book addresses the need for a comprehensive book on the design, synthesis, and characterization of synthetic carbohydrate-based polymeric materials along with their biological applications. The first two chapters cover the synthesis and self-assembly of glycopolymers and different techniques for creating these synthetic polymers. Subsequent chapters account for the preparation of block copolymers, branched glycopolymers, glycosurfaces, glycodendrimers, cationic glycopolymers, bioconjugates, glyconanoparticles and hydrogels. While these chapters comprehensively review the synthetic and characterization methods of those carbohydrate-based materials, their biological applications are discussed in detail.

Bioassay Techniques for Drug Development

Bioassay Techniques for Drug Development
Author : Atta-ur-Rahman,M. Iqbal Choudhary,William J. Thomsen
Publisher : CRC Press
Release Date : 2001-10-04
Category : Medical
Total pages :240
GET BOOK

The goal of an activity-directed isolation process is to isolate bioactive compounds which may provide structural leads of therapeutic importance. Whereas the traditional process of drug development is long and expensive, simple and rapid bioassays can serve as the starting point for drug discovery. This book presents a range of "bench top" bioassa

Biomedical Applications of Nanotechnology

Biomedical Applications of Nanotechnology
Author : Vinod Labhasetwar,Diandra L. Leslie-Pelecky
Publisher : John Wiley & Sons
Release Date : 2007-09-28
Category : Science
Total pages :272
GET BOOK

An overview of nanotechnology and its potential The field of nanotechnology is undergoing rapid developments on many fronts. This reference provides a comprehensive review of various nanotechnologies with a view to their biomedical applications. With chapters contributed by distinguished scientists from diverse disciplines, Biomedical Applications of Nanotechnology : Reviews recent advances in the designing of various nanotechnologies based on nucleic acids, polymers, biomaterials, and metals Discusses biomedical nanotechnology in areas such as drug and gene delivery Covers advanced aspects of imaging and diagnostics Includes a chapter on the issue of nanotoxicology Complete with figures and tables, this is a practical, hands-on reference book for researchers in pharmaceutical and biotech industries, biomedical engineers, pharmaceutical scientists, pharmacologists, and materials scientists as well as for the policymakers who need to understand the potential of nanotechnology. It is also an excellent resource book for graduate-level students in pharmaceutical sciences, biomedical engineering, and other fields in which nanotechnology is playing an increasingly important role.

Enzyme Immunoassays

Enzyme Immunoassays
Author : S.S. Deshpande
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
Category : Science
Total pages :464
GET BOOK

This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Nanoanalytics

Nanoanalytics
Author : Sergei Shtykov
Publisher : Walter de Gruyter GmbH & Co KG
Release Date : 2018-03-19
Category : Science
Total pages :463
GET BOOK

Nanoanalytics is a novel branch of analytical chemistry which explores applications of nanotechnologies in chemical analysis. This comprehensive publication gives an overview of the analytical techniques used to study nanoobjects and nanoparticles as well as the application of nanomaterials themselves in the development of new methods of analysis. The authors also address important metrology aspects and give future prospects of the area.

Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems

Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems
Author : Mohammed Zourob,Sauna Elwary,Anthony P. F. Turner
Publisher : Springer Science & Business Media
Release Date : 2008-09-03
Category : Technology & Engineering
Total pages :970
GET BOOK

Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems will cover the up-to-date biosensor technologies used for the detection of bacteria. Written by the world's most renowned and learned scientists each in their own area of expertise, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems is the first title to cover this expanding research field.