January 21, 2021

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3D Bioprinting

3D Bioprinting
Author : Dong-Woo Cho,Byoung Soo Kim,Jinah Jang,Ge Gao,Wonil Han,Narendra K. Singh
Publisher : Springer Nature
Release Date : 2019-12-04
Category : Medical
Total pages :114
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This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.

3D Bioprinting

3D Bioprinting
Author : Ibrahim Tarik Ozbolat
Publisher : Academic Press
Release Date : 2016-11-21
Category : Technology & Engineering
Total pages :356
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3D Bioprinting: Fundamentals, Principles and Applications provides the latest information on the fundamentals, principles, physics, and applications of 3D bioprinting. It contains descriptions of the various bioprinting processes and technologies used in additive biomanufacturing of tissue constructs, tissues, and organs using living cells. The increasing availability and decreasing costs of 3D printing technologies are driving its use to meet medical needs, and this book provides an overview of these technologies and their integration. Each chapter discusses current limitations on the relevant technology, giving future perspectives. Professor Ozbolat has pulled together expertise from the fields of bioprinting, tissue engineering, tissue fabrication, and 3D printing in his inclusive table of contents. Topics covered include raw materials, processes, machine technology, products, applications, and limitations. The information in this book will help bioengineers, tissue and manufacturing engineers, and medical doctors understand the features of each bioprinting process, as well as bioink and bioprinter types. In addition, the book presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics. Describes all aspects of the bioprinting process, from bioink processing through design for bioprinting, bioprinting techniques, bioprinter technologies, organ printing, applications, and future trends Provides a detailed description of each bioprinting technique with an in-depth understanding of its process modeling, underlying physics and characteristics, suitable bioink and cell types printed, and major accomplishments achieved thus far Explains organ printing technology in detail with a step-by-step roadmap for the 3D bioprinting of organs from isolating stem cells to the post-transplantation of organs Presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics

Bioprinting

Bioprinting
Author : Maika G. Mitchell
Publisher : Academic Press
Release Date : 2017-02-17
Category : Science
Total pages :154
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Innovation is added value to a known process. Bioprinting: Techniques and Risks for Regenerative Medicine aims to stimulate a scientifically grounded, interdisciplinary, multiscale debate and exchange of ideas using the techniques described in the book. 3D printing and additive manufacturing evolved from within the field of Cell Biology will have the ability to recreate cells queried from large amounts of phenotypic and molecular data. Stem Cell biologists, biotechnologists and material engineers, as well as graduate students will greatly benefit from the practical knowledge and case examples provided throughout this book. Shows the possible risk of rejection of 3D printed cells. Contains bioprinting techniques in literature plus actual 3D files adapted and created by the author using several types of 3d printers Provides information on how to convert an existing 3-D printer to bioprinter using currently available techniques Describes the increased complexity of bioprinting compared to 3D- printing Discussion on how 3D printing and additive manufacturing offers the opportunity to 3D print an entire organ, reducing the associated costs of this process when using cells as bioink

3D Bioprinting

3D Bioprinting
Author : Ibrahim Tarik Ozbolat
Publisher : Academic Press
Release Date : 2016-11-21
Category : Technology & Engineering
Total pages :356
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3D Bioprinting: Fundamentals, Principles and Applications provides the latest information on the fundamentals, principles, physics, and applications of 3D bioprinting. It contains descriptions of the various bioprinting processes and technologies used in additive biomanufacturing of tissue constructs, tissues, and organs using living cells. The increasing availability and decreasing costs of 3D printing technologies are driving its use to meet medical needs, and this book provides an overview of these technologies and their integration. Each chapter discusses current limitations on the relevant technology, giving future perspectives. Professor Ozbolat has pulled together expertise from the fields of bioprinting, tissue engineering, tissue fabrication, and 3D printing in his inclusive table of contents. Topics covered include raw materials, processes, machine technology, products, applications, and limitations. The information in this book will help bioengineers, tissue and manufacturing engineers, and medical doctors understand the features of each bioprinting process, as well as bioink and bioprinter types. In addition, the book presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics. Describes all aspects of the bioprinting process, from bioink processing through design for bioprinting, bioprinting techniques, bioprinter technologies, organ printing, applications, and future trends Provides a detailed description of each bioprinting technique with an in-depth understanding of its process modeling, underlying physics and characteristics, suitable bioink and cell types printed, and major accomplishments achieved thus far Explains organ printing technology in detail with a step-by-step roadmap for the 3D bioprinting of organs from isolating stem cells to the post-transplantation of organs Presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics

3D Bioprinting in Regenerative Engineering

3D Bioprinting in Regenerative Engineering
Author : Ali Khademhosseini,Gulden Camci-Unal
Publisher : CRC Press
Release Date : 2018-04-17
Category : Technology & Engineering
Total pages :385
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This book is comprehensive in nature with contributions by leading world experts in 3D bioprinting related to regenerative engineering. It includes history, incorporating the process and methods used in bioprinting. Significant sections will be reserved for the applications of the types of tissues generated by using bioprinting, along with an overview of different technologies used in bioprinting. In addition to equipment, the book also describes the different biomaterials and cells used in these approaches. Overall this is a book that includes both entry-level knowledge and advanced methods and techniques. Applications will emphasize engineering and clinical principles.

3D Bioprinting in Medicine

3D Bioprinting in Medicine
Author : Murat Guvendiren
Publisher : Springer
Release Date : 2019-08-02
Category : Medical
Total pages :209
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This book provides current and emerging developments in bioprinting with respect to bioprinting technologies, bioinks, applications, and regulatory pathways. Topics covered include 3D bioprinting technologies, materials such as bioinks and bioink design, applications of bioprinting complex tissues, tissue and disease models, vasculature, and musculoskeletal tissue. The final chapter is devoted to clinical applications of bioprinting, including the safety, ethical, and regulatory aspects. This book serves as a go-to reference on bioprinting and is ideal for students, researchers and professionals, including those in academia, government, the medical industry, and healthcare.

3D Bioprinting for Reconstructive Surgery

3D Bioprinting for Reconstructive Surgery
Author : Daniel Thomas,Zita M. Jessop,Iain S. Whitaker
Publisher : Woodhead Publishing
Release Date : 2017-11-14
Category : Technology & Engineering
Total pages :450
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3D Bioprinting for Reconstructive Surgery: Techniques and Applications examines the combined use of materials, procedures and tools necessary for creating structural tissue constructs for reconstructive purposes. Offering a broad analysis of the field, the first set of chapters review the range of biomaterials which can be used to create 3D-printed tissue constructs. Part Two looks at the techniques needed to prepare biomaterials and biological materials for 3D printing, while the final set of chapters examines application-specific examples of tissues formed from 3D printed biomaterials. 3D printing of biomaterials for tissue engineering applications is becoming increasingly popular due to its ability to offer unique, patient-specific parts—on demand—at a relatively low cost. This book is a valuable resource for biomaterials scientists, biomedical engineers, practitioners and students wishing to broaden their knowledge in the allied field. Discusses new possibilities in tissue engineering with 3D printing Presents a comprehensive coverage of the materials, techniques and tools needed for producing bioprinted tissues Reviews emerging technologies in addition to commercial techniques

3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine

3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine
Author : Lijie Grace Zhang,John P Fisher,Kam Leong
Publisher : Academic Press
Release Date : 2015-01-14
Category : Technology & Engineering
Total pages :392
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3D Bioprinting and Nanotechnology in Tissue Engineering provides an in depth introduction to these two technologies and their industrial applications. Stem cells in tissue regeneration are covered, along with nanobiomaterials. Commercialization, legal and regulatory considerations are also discussed in order to help you translate nanotechnology and 3D printing-based products to the marketplace and the clinic. Dr. Zhang’s and Dr. Fishers’ team of expert contributors have pooled their expertise in order to provide a summary of the suitability, sustainability and limitations of each technique for each specific application. The increasing availability and decreasing costs of nanotechnologies and 3D printing technologies are driving their use to meet medical needs, and this book provides an overview of these technologies and their integration. It shows how nanotechnology can increase the clinical efficiency of prosthesis or artificial tissues made by bioprinting or biofabrication. Students and professionals will receive a balanced assessment of relevant technology with theoretical foundation, while still learning about the newest printing techniques. Includes clinical applications, regulatory hurdles, and risk-benefit analysis of each technology. This book will assist you in selecting the best materials and identifying the right parameters for printing, plus incorporate cells and biologically active agents into a printed structure Learn the advantages of integrating 3D printing and nanotechnology in order to improve the safety of your nano-scale materials for biomedical applications

3D Bioprinting

3D Bioprinting
Author : Jeremy M. Crook
Publisher : Humana
Release Date : 2020-03-24
Category : Science
Total pages :260
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This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

Extrusion Bioprinting of Scaffolds for Tissue Engineering Applications

Extrusion Bioprinting of Scaffolds for Tissue Engineering Applications
Author : Daniel X. B. Chen
Publisher : Springer
Release Date : 2018-12-13
Category : Technology & Engineering
Total pages :171
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This book introduces readers to the theory and practice of extrusion bio-printing of scaffolds for tissue engineering applications. The author emphasizes the fundamentals and practical applications of extrusion bio-printing to scaffold fabrication, in a manner particularly suitable for those who wish to master the subject matter and apply it to real tissue engineering applications. Readers will learn to design, fabricate, and characterize tissue scaffolds to be created by means of extrusion bio-printing technology.

Bioprinting in Regenerative Medicine

Bioprinting in Regenerative Medicine
Author : Kursad Turksen
Publisher : Springer
Release Date : 2015-09-02
Category : Science
Total pages :140
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This volume presents the current state of laser-assisted bioprinting, a cutting edge tissue engineering technology. Nineteen chapters discuss the most recent developments in using this technology for engineering different types of tissue. Beginning with an overview, the discussion covers bioprinting in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Because bioprinting is on its way to becoming a dominant technology in tissue-engineering, Bioprinting in Regenerative Medicine is essential reading for those researching or working in regenerative medicine, tissue engineering or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable.

Study on Microextrusion-based 3D Bioprinting and Bioink Crosslinking Mechanisms

Study on Microextrusion-based 3D Bioprinting and Bioink Crosslinking Mechanisms
Author : Liliang Ouyang
Publisher : Springer
Release Date : 2019-08-10
Category : Technology & Engineering
Total pages :129
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This book presents a comprehensive study on microextrusion-based 3D bioprinting technologies for bioinks with various crosslinking mechanisms, chiefly focusing on the bioprinting process and bioink properties to provide readers with a better understanding of this state-of-the-art technology. Further, it summarizes a number of general criteria and research routes for microextrusion-based 3D bioprinting using three experimental studies based on shear-thinning, thermo-sensitive and non-viscous hydrogel bioinks. The book also presents sample applications in the areas of stem cells and cell matrix interaction. The book highlights pioneering results in the development of bioprinting technologies and bioinks, which were published in high-quality journals such as Advanced Materials, Biofabrication and ACS Biomaterials Science & Engineering. These include an in-situ crosslinking strategy that overcomes the viscosity limits for bioinks, which is virtually impossible using conventional strategies, and can be generalized for other bioink formulations.

Exam Prep for: 3d Bioprinting for Reconstructive Surgery

Exam Prep for: 3d Bioprinting for Reconstructive Surgery
Author : Anonim
Publisher : Unknown
Release Date : 2021
Category :
Total pages :129
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In Situ Bioprinting of Cell-laden Gelma Scaffolds for the Treatment of Skeletal Muscle Defects

In Situ Bioprinting of Cell-laden Gelma Scaffolds for the Treatment of Skeletal Muscle Defects
Author : Carina S. Russell
Publisher : Unknown
Release Date : 2019
Category :
Total pages :129
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issue engineered scaffolds, reconstructive surgery, and bracing remain inadequate for the treatment of skeletal muscle defects, especially for volumetric muscle loss (VML). Skeletal muscle defects in VML varies on a case-by-case basis, most often in irregular and unpredictable geometries. Three-dimensional (3D) printing has emerged as one strategy that enables the fabrication of scaffolds that match the geometry of the defect site. However, with traditional 3D printing the time and facilities needed for imaging the defect site, post-processing to render computer-aided models, and printing a suitable scaffold prevent immediate reconstructive interventions post-traumatic injuries. In addition, the proper implantation of hydrogel-based scaffolds, which have generated promising results in vitro, is a significant challenge. To overcome these challenges, a new treatment paradigm is proposed in which adhesive gelatin-based hydrogels are printed directly into the defect area and photocrosslinked in situ. The overarching objective was to determine the proper gelatin methacryloyl (GelMA) concentration that exhibited the correct mechanical and physiochemical attributes to facilitate myogenesis. The printability was optimized to ensure continuous fiber deposition and that no back pressure build up from the nozzle could adversely affect the printing of complex structures. The adhesiveness of the bioink hydrogel to the skeletal muscles was assessed ex vivo to confirm that the hydrogel scaffold could adhere to skeletal muscle directly at a defect site. Cellular morphology and the promotion of myogenesis was inspected by staining. The suitability of the in situ bioprinted GelMA hydrogel scaffolds was successfully assessed in vitro for the encapsulation and delivery of skeletal muscle myoblasts. In addition, acellular gelatin-based hydrogel scaffolds were bioprinted in vivo onto a critical VML injury of 11-week old C57/Bl6 mice to examine inflammation, fibrosis formation, rupturing at the scaffold-muscle interface and promotion of remnant skeletal muscle hypertrophy. The developed portable bioprinter capable of 3D in situ printing of adhesive scaffolds successfully demonstrated precise filling of complex skeletal muscle tissue defects.

Biomimetic Medical Materials

Biomimetic Medical Materials
Author : Insup Noh
Publisher : Springer
Release Date : 2018-11-23
Category : Medical
Total pages :399
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This volume outlines the current status in the field of biomimetic medical materials and illustrates research into their applications in tissue engineering. The book is divided into six parts, focusing on nano biomaterials, stem cells, tissue engineering, 3D printing, immune responses and intellectual property. Each chapter has its own introduction and outlines current research trends in a variety of applications of biomimetic medical materials. The biomimetic medical materials that are covered include functional hydrogels, nanoparticles for drug delivery and medicine, the 3D bioprinting of biomaterials, sensor materials, stem cell interactions with biomaterials, immune responses to biomaterials, biodegradable hard scaffolds for tissue engineering, as well as other important topics, like intellectual property. Each chapter is written by a team of experts. This volume attempts to introduce the biomimetic properties of biomedical materials within the context of our current understanding of the nanotechnology of nanoparticles and fibres and the macroscopic aspects of 3D bioprinting.