November 25, 2020

Download Ebook Free Forced-Flow Layer Chromatography

Forced-Flow Layer Chromatography

Forced-Flow Layer Chromatography
Author : Erno Tyihak
Publisher : Elsevier
Release Date : 2015-12-01
Category : Science
Total pages :524
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Forced-Flow Layer Chromatography takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective. This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis. Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spots Emphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years ago Discusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances

Thin Layer Chromatography in Phytochemistry

Thin Layer Chromatography in Phytochemistry
Author : Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska
Publisher : CRC Press
Release Date : 2008-03-04
Category : Science
Total pages :896
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Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Instrumental Thin-Layer Chromatography

Instrumental Thin-Layer Chromatography
Author : Colin Poole
Publisher : Elsevier
Release Date : 2014-09-22
Category : Science
Total pages :652
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Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists. Contributions written by recognized authoritative and visionary experts Focuses on state-of-the-art instrumental thin-layer chromatography and advanced applications across many areas Provides guidance on the analysis of complex, dirty mixtures of compounds Offers a cost-effective analytic technique for laboratories working under strict budgets

Thin-Layer Chromatography

Thin-Layer Chromatography
Author : Peter E Wall
Publisher : Royal Society of Chemistry
Release Date : 2007-10-31
Category : Science
Total pages :204
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Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.

Handbook of Thin-Layer Chromatography

Handbook of Thin-Layer Chromatography
Author : Joseph Sherma,Bernard Fried
Publisher : CRC Press
Release Date : 2003-04-18
Category : Science
Total pages :1048
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In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

Instrumental Thin-Layer Chromatography

Instrumental Thin-Layer Chromatography
Author : Colin Poole
Publisher : Elsevier
Release Date : 2014-09-22
Category : Science
Total pages :652
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Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists. Contributions written by recognized authoritative and visionary experts Focuses on state-of-the-art instrumental thin-layer chromatography and advanced applications across many areas Provides guidance on the analysis of complex, dirty mixtures of compounds Offers a cost-effective analytic technique for laboratories working under strict budgets

The Essence of Chromatography

The Essence of Chromatography
Author : Colin F. Poole
Publisher : Elsevier
Release Date : 2003
Category : Science
Total pages :925
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General concepts in column chromatography -- The column in gas chromatography -- Instrumental aspects of gas chromatography -- The column in liquid chromatography -- Instrumental aspects of liquid chromatography -- Thin-layer chromatography -- Supercritical fluid chromatography -- Capillary-electromigration separation techniques -- Spectroscopic detectors for identification and quantification -- Separation of stereoisomers -- Laboratory-scale preparative chromatography.

Practical Thin-Layer Chromatography

Practical Thin-Layer Chromatography
Author : Bernard Fried,Joseph Sherma
Publisher : CRC Press
Release Date : 1996-05-29
Category : Science
Total pages :288
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Practical Thin-Layer Chromatography provides thorough coverage of the principles, practices, and applications of thin-layer chromatography (TLC) for important sample and compound types. This information is directed specifically at workers in the most active scientific fields.

Planar Chromatography

Planar Chromatography
Author : Szabolcs Nyiredy
Publisher : Unknown
Release Date : 2001
Category : Chromatographic analysis
Total pages :614
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Development And Validation Of Chromatographic Methods For Simultaneous Quantification Of Drugs In Bulk And In Their Formulations: HPLC And HPTLC Techniques

Development And Validation Of Chromatographic Methods For Simultaneous Quantification Of Drugs In Bulk And In Their Formulations: HPLC And HPTLC Techniques
Author : Satish Y. Gabhe,Kakasaheb R. Mahadik,Sachin E. Potawale,Anirudh B. Thati
Publisher : Anchor Academic Publishing (aap_verlag)
Release Date : 2014-08
Category : Business & Economics
Total pages :112
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This book details: 1. Development and validation of a HPTLC-densitometric method for concurrent estimation of metformin hydrochloride, pioglitazone hydrochloride and gliclazide in combined dosage form. 2. Development and validation of a HPTLC method for simultaneous estimation of moxifloxacin hydrochloride and dexamethasone sodium phosphate in combined pharmaceutical dosage form. 3. Development and validation of a RP-HPLC method for simultaneous estimation of ciprofloxacin hydrochloride and dexamethasone in combined dosage form, which is a better alternative to existing ones. The developed analytical methods are simple, selective, accurate, robust, and precise with shorter analysis time for the analysis of drug/s in combined pharmaceutical dosage forms. All the developed HPTLC and HPLC methods have been validated as per ICH Q2 (R1) guideline. Developed analytical methods could boost analytical researchers to work more efficiently in the field of analytical method development and validation of Pharmaceutical dosage forms.

High-Performance Thin-Layer Chromatography (HPTLC)

High-Performance Thin-Layer Chromatography (HPTLC)
Author : ManMohan Srivastava
Publisher : Springer Science & Business Media
Release Date : 2010-11-15
Category : Science
Total pages :397
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The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.

Planar Chromatography - Mass Spectrometry

Planar Chromatography - Mass Spectrometry
Author : Teresa Kowalska,Mieczyslaw Sajewicz,Joseph Sherma
Publisher : CRC Press
Release Date : 2015-11-18
Category : Medical
Total pages :380
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Planar Chromatography–Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography–mass spectrometry as a hyphenated tool of analytical chemistry. It also examines the high and as-yet unexploited potential of planar chromatography–mass spectrometry for analytical use in scientific investigations. This book overviews the combination of planar chromatography, a relatively simple and cost-effective separation step for determining complex mixtures of compounds, with mass spectrometry, an efficient, highly instrumental, and relatively expensive technique that enables rapid identification of separated chemical species. It covers electrophoretic–mass spectrometry methods and applications, which are considered planar chromatographic techniques and are increasingly being exploited in proteomic and molecular biology studies as well as for medical diagnostic purposes. It also provides a selection of applications, such as drug control and forensic and food analysis, including more difficult substances such as carbohydrates and lipids. The book advocates growth in using planar chromatography–mass spectrometry in laboratories that have appropriate equipment but have not yet employed the techniques in combination. It also describes the use of a relatively inexpensive commercial system that can be adopted by laboratories currently working without the coupled methodology. Aiming to improve power and efficiency when other analytical methods are inadequate, Planar Chromatography–Mass Spectrometry encourages separation science practitioners in academia and industry to combine the two methods for enhanced results.

Mass Spectrometry Handbook

Mass Spectrometry Handbook
Author : Mike S. Lee
Publisher : John Wiley & Sons
Release Date : 2012-04-16
Category : Science
Total pages :1368
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Due to its enormous sensitivity and ease of use, mass spectrometry has grown into the analytical tool of choice in most industries and areas of research. This unique reference provides an extensive library of methods used in mass spectrometry, covering applications of mass spectrometry in fields as diverse as drug discovery, environmental science, forensic science, clinical analysis, polymers, oil composition, doping, cellular research, semiconductor, ceramics, metals and alloys, and homeland security. The book provides the reader with a protocol for the technique described (including sampling methods) and explains why to use a particular method and not others. Essential for MS specialists working in industrial, environmental, and clinical fields.

Chromatography and Isolation of Insect Hormones and Pheromones

Chromatography and Isolation of Insect Hormones and Pheromones
Author : A.R. McCaffery,I.D. Wilson
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
Category : Science
Total pages :390
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The connection between the study of insects. their development. behaviour and biochemistry. and chromatography is perhaps not immediately obvious. However. this connection exists and it is of fundamental importance to our understanding of many areas of insect physiology. Insects range in size from small to minute and consequently the amounts of hormones or pheromones they produce are equally minute. Ultimately any attempt at understanding the processes which control development, social behaviour or the biochemistry of insects requires some means of isolating the tiny quantities of the hormones and pheromones responsible in sufficient quantity and purity for identification. The ability to devise novel techniques to separate these materials from frequently complex biological mixtures including precursors and metabolites and to devise detection systems for them is vital. Methods for the quantification of these substances at different stages in the life cycle. or in response to environmental change or stress. are then essential. Chromatography. both as a means for isolation and as a method for quantitative analysis. has "been an essential tool in these studies. This volume represents the outcome of a joint international symposium organized by the Chromatographic and Royal Entomological Societies at the University of Reading between the 21st and 23rd March 1989 aimed specifically at discussing the chromatography and isolation of insect hormones. pheromones and related substances.The papers presented at that meeting. and collected together here. covered many aspects of the subject including the chromatography of juvenile hormones. ecdysteroids. peptides. pheromones and semio chemicals.

Handbook Of Thin-Layer Chromatography, Second Edition

Handbook Of Thin-Layer Chromatography, Second Edition
Author : Joseph Sherma,Bernard Fried
Publisher : CRC Press
Release Date : 1996-03-14
Category : Science
Total pages :1104
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Written by over 40 internationally acclaimed authorities on thin-layer chromatography (TLC), this comprehensive Second Edition presents the latest techniques, instrumentation, and applications of overpressurized, rotational, and high-performance quantitative TLC. Offering a systematic approach to TLC, the Handbook of Thin-Layer Chromatography, Second Edition contains new, practical information on the detection, identification, and documentation of chromatograph zones...optical quantitation...flame ionization detection...automation and robotics...nucleic acid derivatives...and more.