December 1, 2020

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Human Stem Cell Manual

Human Stem Cell Manual
Author : Jeanne F. Loring,Suzanne Peterson
Publisher : Academic Press
Release Date : 2012
Category : Medical
Total pages :619
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This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses. • Offers a comprehensive introduction to stem cell biology and culture for medical and biology researchers investigating diagnostics and treatments for various diseases • Presents a historical introduction, discussion of alternative methods, and common pitfalls for basic and advanced experimental strategies • Includes new chapters devoted to iPS cells and other alternative sources for generating human stem cells written by the scientists who made these breakthroughs

Human Stem Cell Manual

Human Stem Cell Manual
Author : Jeanne Frances Loring
Publisher : Elsevier
Release Date : 2007
Category : Medical
Total pages :461
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Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

Human Stem Cell Technology and Biology

Human Stem Cell Technology and Biology
Author : Gary S. Stein,Maria Borowski,Mai X. Luong,Meng-Jiao Shi,Kelly P. Smith,Priscilla Vazquez
Publisher : John Wiley & Sons
Release Date : 2011-03-04
Category : Science
Total pages :440
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Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Human Stem Cell Manual

Human Stem Cell Manual
Author : Suzanne Peterson,Jeanne F. Loring,Robin L. Wesselschmidt,Philip H. Schwartz
Publisher : Elsevier
Release Date : 2011-10-10
Category : Medical
Total pages :488
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Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

Manual of Stem Cell and Bone Marrow Transplantation

Manual of Stem Cell and Bone Marrow Transplantation
Author : Joseph H. Antin,Deborah Yolin Raley
Publisher : Cambridge University Press
Release Date : 2013-10-17
Category : Medical
Total pages :202
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The fully revised second edition is a practical manual for all members of the stem cell and bone marrow transplant team.

A Manual for Differentiation of Bone Marrow-Derived Stem Cells to Specific Cell Types

A Manual for Differentiation of Bone Marrow-Derived Stem Cells to Specific Cell Types
Author : Gilson Khang,Tatsuya Shimizu,Kee Woei NgSeries
Publisher : World Scientific Publishing Company
Release Date : 2014-02-11
Category : Science
Total pages :212
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This is the first experimental protocol book that covers the differentiation of bone marrow-derived stem cells (BMSCs) into specific cell types, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds. BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost any kind of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissues such as blood vessels, heart, liver and so on. For the differentiation of BMSCs, many methods have been introduced to adjust their microenvironment (chemical and physical cues), including chemical induction methods using large or small molecules and pellet culture; mechanical stimulation induction methods using cyclic mechano-transduction or ultrasonication; cytokine-released method using scaffolds; and so on.

Culture of Human Stem Cells

Culture of Human Stem Cells
Author : R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach
Publisher : John Wiley & Sons
Release Date : 2007-07-16
Category : Science
Total pages :360
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This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Stem Cell Biology

Stem Cell Biology
Author : Dr. Indumathi Somasundaram,Dr. Dhanasekaran Marappagounder,Dr. Pankaj Kaingade
Publisher : Evincepub Publishing
Release Date : 2020-01-18
Category : Science
Total pages :102
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Stem cell research and therapeutics has been captivating in recent decades due to its versatile discoveries on its potential to treat myriad of diseases. This is due to its properties of being undifferentiated, yet self-renews and differentiates into any type of cells, upon physiological signaling mechanisms. These sole attributes of stem cells, has created inquisitiveness in the minds of scientists and young researchers to explore every aspects of stem cells. This has opened a single doorway to the world of regenerative medicine, thereby saving millions of lives from wide diseases. Although stem cell is creating a new revolution in recent decades, due to its wider hypes and advancement in technologies, it is not becoming cost-effective for young generation University/college students to actually understand what stem cells are? Now-a-days, almost every life science course in College/ University either UG, PG or Specialized Diploma, has either a Unit on Stem cell or a full subject/paper on stem cell biology. Unfortunately, although learning about this new emerging field is imperative, academicians are unable to fulfill the stem cell practical due to the hype created about its tedious, costly coupled with advanced instrumentation facility. Thus, youngsters, being the future scientists are unaware about basic techniques of stem cell biology. Wide job opportunities are increasing day by day for youngsters in biotech industries, healthcare sectors, stem cell companies and banking sectors. Nevertheless, the students are unable to make the best use of these jobs, due to lack of basic know-how on stem cell biology or their basic laboratory techniques. This book is created to remove the bottleneck involved and help students to learn all the stem cell laboratory technique in a simple and easy fashion. We explain over here, the practical manual stem cell techniques that can be done with a basic culture laboratory set up. With such simple manual techniques coupled with shorter time and cost-effectiveness, students can understand all underlying principles and procedures involved in collection, isolation, enumeration, culturing, differentiation and in-vitro characterization of stem cells from various adult stem cell sources in a step-wise easy to understand manner. Although flowcytometry plays an important role in characterization of stem cells, due to cost of the machine, techniques of flowcytometry cannot be a part of lab manual in course of various Universities. However, to help the students, the authors are soon coming out with a new book on "Characterization of stem cells using flowcytometry: An easy Guide".

Handbook of Stem Cells

Handbook of Stem Cells
Author : Anthony Atala,Robert Lanza
Publisher : Academic Press
Release Date : 2012-12-31
Category : Science
Total pages :1074
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New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity. The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the requisites for a general understanding of adult and embryonic stem cells. Organized in two volumes entitled Pluripotent Stem Cells and Cell Biology and Adult and Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a the latest information of what is known about each specific organ system. Provides comprehensive coverage on this highly topical subject Contains contributions by the foremost authorities and premiere names in the field of stem cell research Companion website - http://booksite.elsevier.com/9780123859426/ - contains over 250 color figures in presentation format

Pluripotent Stem Cells

Pluripotent Stem Cells
Author : Minoru Tomizawa
Publisher : BoD – Books on Demand
Release Date : 2016-07-20
Category : Science
Total pages :532
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Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.

The Biology and Therapeutic Application of Mesenchymal Cells - Set

The Biology and Therapeutic Application of Mesenchymal Cells - Set
Author : Kerry Atkinson
Publisher : John Wiley & Sons
Release Date : 2017-01-17
Category : Medical
Total pages :1048
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The Biology and Therapeutic Application of Mesenchymal Cells comprehensively describes the cellular and molecular biology of mesenchymal stem cells and mesenchymal stromal cells, describing their therapeutic potential in a wide variety of preclinical models of human diseases and their mechanism of action in these preclinical models. Chapters also discuss the current status of the use of mesenchymal stem and stromal cells in clinical trials in a wide range of human diseases and disorders, for many of which there are limited, or no other, therapeutic avenues. • Provides coverage on both the biology of mesenchymal stem cells and stromal cells, and their therapeutic applications • Describes the therapeutic potential of mesenchymal stem and stromal cells in a wide variety of preclinical models of human diseases and their mechanism of action in these preclinical models • Discusses the current status of mesenchymal stem and stromal cells in clinical trials in a wide range of human diseases and disorders, for many of which there are limited, or no other, therapeutic avenues • Written and edited by leaders in the field The Biology and Therapeutic Application of Mesenchymal Cells is an invaluable resource for those studying stem cells, cell biology, genetics, gene or cell therapy, or regenerative medicine. About the Author Kerry Atkinson, MBBS MD DTM&H FRCP FRACP, is an Adjunct Professor at the University of Queensland Centre for Clinical Research in Brisbane, Australia, an Adjunct Professor in the Stem Cell Laboratories, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia and a Specialist in Internal Medicine at the Salisbury Medical Centre, Brisbane, Queensland, Australia.

Stem Cells Handbook

Stem Cells Handbook
Author : Stewart Sell
Publisher : Springer Science & Business Media
Release Date : 2013-08-16
Category : Science
Total pages :534
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This book discusses critical areas of progress in stem cell research, including the most recent research and applications of pluripotent embryonic cells, induced pluripotent cells, oligopotent tissue stem cells and cancer stem cells. The text covers basic knowledge of stem cell biology, stem cell ethics, development of techniques for applying stem cell therapy, the technology of obtaining appropriate cells for transplantation as well as the role of stem cells in cancer and how therapy may be directed to cancer stem cells. This new volume is essential reading for all scientists currently in the field or allied research areas, and those for those graduate students who envision a career in stem cells.

Embryonic Stem Cell Protocols

Embryonic Stem Cell Protocols
Author : Kursad Turksen
Publisher : Springer Science & Business Media
Release Date : 2006
Category : CD-ROMs
Total pages :500
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It is clear that the potentials of assessing embryonic stem (ES) cells in regenerative medicine applications is evident in the ever-increasing publications in which ES cell biology and differentiation along diverse lineages appear in the academic as well as the popular press. These two new volumes present important advances in the field since the publication of Embryonic Stem Cells: Methods and Protocols four years ago. These two volumes provide an update and complement to that volume, focusing on ES cells recently isolated from other/non-mouse species. Each volume contains numerous updates, more advanced approaches; and completely new protocols for the use of ES cells in studies of diverse cell lineages. These two volumes will surely expand the experimental repertoires of both experts and novices in the field.

Culture of Animal Cells

Culture of Animal Cells
Author : R. Ian Freshney
Publisher : Wiley-Liss
Release Date : 1993-12-29
Category : Science
Total pages :510
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This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Tissue Engineering

Tissue Engineering
Author : Clemens Van Blitterswijk,Jan De Boer
Publisher : Academic Press
Release Date : 2014-12-10
Category : Technology & Engineering
Total pages :896
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Tissue Engineering is a comprehensive introduction to the engineering and biological aspects of this critical subject. With contributions from internationally renowned authors, it provides a broad perspective on tissue engineering for students coming to the subject for the first time. In addition to the key topics covered in the previous edition, this update also includes new material on the regulatory authorities, commercial considerations as well as new chapters on microfabrication, materiomics and cell/biomaterial interface. Effectively reviews major foundational topics in tissue engineering in a clear and accessible fashion Includes state of the art experiments presented in break-out boxes, chapter objectives, chapter summaries, and multiple choice questions to aid learning New edition contains material on regulatory authorities and commercial considerations in tissue engineering