November 27, 2020

Download Ebook Free Introductory Experiments On Biomolecules And Their Interactions

Introductory Experiments on Biomolecules and their Interactions

Introductory Experiments on Biomolecules and their Interactions
Author : Robert K. Delong,Qiongqiong Zhou
Publisher : Academic Press
Release Date : 2015-03-06
Category : Science
Total pages :126
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Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. Features quantitative analysis of RNA degradation by RNase Contains problem sets, calculations, and references for each lab fully immersing students in the learning process Includes instruction on how to maintain a lab notebook and write a formal lab report Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions

An Introduction to Single Molecule Biophysics

An Introduction to Single Molecule Biophysics
Author : Yuri L. Lyubchenko
Publisher : CRC Press
Release Date : 2017-11-22
Category : Science
Total pages :240
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This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications. Covers the most widely used single molecule biophysics techniques Provides numerous examples of current biological applications Includes practical tips on measurement optimization, resolution limits, etc. Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center.

Metallointercalators

Metallointercalators
Author : Janice Aldrich-Wright
Publisher : Springer
Release Date : 2011-04-06
Category : Science
Total pages :427
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A comprehensive treatment of the characterisation techniques used in investigating inorganic and organic molecules that interact with biomolecules is presented to the reader in a clear fashion. The work consists of two parts: (i) synthetic aspects of metallointercalators along with targeting and improving transport and (ii) the various techniques that are used for probing their interactions, such as; DNA-NMR, PGSE-NMR, DNA ESI-MS, Linear and Circular Dichroism, Fluorescence Spectroscopy, Confocal Microscopy, Viscosity, TGA and dialysis, Microarrays, biological analysis. Chapters are devoted to the synthesis and the techniques used to study the interactions of inorganic complexes with biomolecules. Considerably detailed examples are used to help illustrate the application of these techniques. This book is a useful resource for an array of inorganic and organic advanced undergraduate and graduate courses and for researchers in drug discovery.

The Smallest Biomolecules: Diatomics and their Interactions with Heme Proteins

The Smallest Biomolecules: Diatomics and their Interactions with Heme Proteins
Author : Abhik Ghosh
Publisher : Elsevier
Release Date : 2011-10-13
Category : Science
Total pages :614
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This is not a book on NO biology, nor about hemoglobin, nor about heme-based sensors per se. Of course, it covers all these topics and more, but above all, it aims at providing a truly multidisciplinary perspective of heme-diatomic interactions. The overarching goal is to build bridges among disciplines, to bring about a meeting of minds. The contributors to this book hail from diverse university departments and disciplines – chemistry, biochemistry, molecular biology, microbiology, zoology, physics, medicine and surgery, bringing with them very different views of heme-diatomic interactions. The hope is that the juxtaposition of this diversity will lead to increased exchanges of ideas, approaches, and techniques across traditional disciplinary boundaries. The authors represent a veritable Who’s Who of heme protein research and include John Olson, Tom Spiro, Walter Zumft, F. Ann Walker, Teizo Kitagawa, W. Robert Scheidt, Pat Farmer, Marie-Alda Gilles-Gonzalez, and many other equally distinguished scientists. Extremely distinguished list of authors Multidisciplinary character – equally suitable for chemists and biochemists Covers the hottest topics in heme protein research: sensors, NO biology, new roles of hemoglobin, etc.

Real-Time Analysis of Biomolecular Interactions

Real-Time Analysis of Biomolecular Interactions
Author : K. Nagata,H. Handa
Publisher : Springer Science & Business Media
Release Date : 2013-12-11
Category : Science
Total pages :256
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The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of BIACORE.

Handbook of Biophotonics

Handbook of Biophotonics
Author : Jürgen Popp,Valery V. Tuchin,Arthur Chiou,Stefan H. Heinemann
Publisher : John Wiley & Sons
Release Date : 2011-11-30
Category : Science
Total pages :1131
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This debut edition of Visualizing Physical Geography encompasses the science of physical geography from local to global scales, using a uniquely visual approach to take students on a journey from the top layers of the Earth’s atmosphere to the rocks underlying the ocean basins to the forests of the farthest continents. As students explore the critical topics of physical geography, their study of the climate, surface features, and habitats around the world uses basic concepts of ecology, geology, chemistry, environmental science, biology, and physics and many other disciplines. Visualizing Physical Geography reinforces these interacting components and, with its premier art program, vividly illustrates the interconnectedness of physical processes that weave together to create our planet’s dynamic surface and atmosphere. Visualizing Geography relies heavily on other visuals with text to elucidate concepts for students and solidify their understanding of them. The goal is to help students understand the world around them and interpret what they see in a meaningful, accurate and exciting way. The content, design and layout of the text takes advantage of the full capacity in which students process information – visual as well as verbal. Visualizing Geography also helps students examine their own personal studying and learning styles with several new pedagogical aids –– encouraging students to apply what they are learning to their everyday lives. Visualizing Geography continues to offer ongoing study tips and psychological techniques for mastering the material. Most importantly students are provided with numerous opportunities to immediately access their understanding.

Molecular Biology of the Cell

Molecular Biology of the Cell
Author : Bruce Alberts
Publisher : Unknown
Release Date : 2004
Category :
Total pages :129
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Biomolecules in Organic Solvents

Biomolecules in Organic Solvents
Author : Armando Gomez-Puyou
Publisher : CRC Press
Release Date : 1992-01-27
Category : Medical
Total pages :288
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Biomolecules and Organic Solvents discusses the behavior of enzymes and multi-enzyme complexes in organic solvents, in addition to the problem of water-protein interactions and the characteristics of water at interphases. It attempts to bridge the gap between the all water and the organic solvents systems from the point of view of enzyme structure and function. The mechanism of action of enzymes dispersed in anhydrous organic solvents and the biotechnological perspectives of the field are evaluated, and new information regarding the function and characteristics of complex multi-enzymatic systems and whole cells in organic solvents are examined. New developments regarding the nature of the water pool and interphases of reverse micelles and their enzyme kinetics are also explored. The characteristics and properties of enzymes trapped in reverse micelles are discussed throughout the book. Biomolecules in Organic Solvents is essential reading for biochemists, molecular biologists, and others working in related fields.

Quantifying Interactions of Biomolecules with Inorganic Surfaces

Quantifying Interactions of Biomolecules with Inorganic Surfaces
Author : Abhijeet Patra
Publisher : Springer
Release Date : 2016-12-01
Category : Science
Total pages :102
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This thesis demonstrates the adaptation of existing techniques and principles towards enabling clean and precise measurements of biomolecules interacting with inorganic surfaces. In particular, it includes real-time measurement of serum proteins interacting with engineered nanomaterial. Making meaningful and unambiguous measurements has been an evolving problem in the field of biology and its various allied domains, primarily due to the complex nature of experiments and the large number of possible interferants. The subsequent quantification of interactions between biomolecules and inorganic surfaces solves pressing problems in the rapidly developing fields of lipidomics and nanomedicine.

Non-covalent Interactions

Non-covalent Interactions
Author : Pavel Hobza,Klaus Müller-Dethlefs
Publisher : Royal Society of Chemistry
Release Date : 2010
Category : Science
Total pages :225
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Co-authored by an experimentalist (Klaus M ller-Dethlefs ) and theoretician (Pavel Hobza), the aim of this book is to provide a general introduction into the science behind non-covalent interactions and molecular complexes using some important experimental and theoretical methods and approaches."

Photophysics of Ionic Biochromophores

Photophysics of Ionic Biochromophores
Author : Steen Brøndsted Nielsen,Jean Ann Wyer
Publisher : Springer Science & Business Media
Release Date : 2013-10-16
Category : Science
Total pages :230
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This book provides a concise overview of the photophysics and spectroscopy of bio chromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states. This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.

Aspects of Physical Biology

Aspects of Physical Biology
Author : Giancarlo Franzese,Miguel Rubi
Publisher : Springer Science & Business Media
Release Date : 2008-07-10
Category : Science
Total pages :233
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The application to Biology of the methodologies developed in Physics is attracting an increasing interest from the scientific community. It has led to the emergence of a new interdisciplinary field, called Physical Biology, with the aim of reaching a better understanding of the biological mechanisms at molecular and cellular levels. Statistical Mechanics in particular plays an important role in the development of this new field. For this reason, the XXth session of the famous Sitges Conference on Statistical Physics was dedicated to "Physical Biology: from Molecular Interactions to Cellular Behavior". As is by now tradition, a number of lectures were subsequently selected, expanded and updated for publication as lecture notes, so as to provide both a state-of-the-art introduction and overview to a number of subjects of broader interest and to favor the interchange and cross-fertilization of ideas between biologists and physicists. The present volume focuses on three main subtopics (biological water, protein solutions as well as transport and replication), presenting for each of them the on-going debates on recent results. The role of water in biological processes, the mechanisms of protein folding, the phases and cooperative effects in biological solutions, the thermodynamic description of replication, transport and neural activity, all are subjects that are revised in this volume, based on new experiments and new theoretical interpretations.

Nanomechanics

Nanomechanics
Author : Alexander Vakhrushev
Publisher : BoD – Books on Demand
Release Date : 2017-05-24
Category : Science
Total pages :194
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In recent years, nanotechnology is the basis for the development of modern production. This determined the urgency of the intensive development of the new direction of mechanics and nanomechanics, for the scientific description of nanotechnological processes and the solution of several topical nanotechnology problems. Topics included in the book cover a wide range of research in the field of nanomechanics: thermomass theory of nanosystems; deformation of nanomaterials; interface mechanics of assembly carbon nanotube; nanomechanics on surface; molecular interactions and transformations; nanomechanical sensors, nanobeams, and micromembranes; nanostructural organic and inorganic materials; green synthesis of metallic nanoparticles. The main goal of these works is the establishment of the nanosystem macroparameter dependence on its nanoparameters using nanomechanics. This book will be useful for engineers, technologists, and researchers interested in methods of nanomechanics and in advanced nanomaterials with complex behavior and their applications.

Biosensors

Biosensors
Author : Jagriti Narang,C.S. Pundir
Publisher : CRC Press
Release Date : 2017-09-19
Category : Medical
Total pages :160
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Nanotechnology is a budding field and has a pivotal role in sensing. Nanomaterials exist in various forms such as nanoparticles, nanoclusters, nanobelts, and nanospheres. These nanomaterials act as sensing interfaces and immobilization surfaces for various biomolecules such as enzymes, DNA, and antigens. Therefore, the preparation and characterization of these nanoparticles play an important role in sensing devices. This handbook has evolved from the authors’ teaching and research experience in the field of nanoparticle biosensing. It encompasses protocols for the synthesis of various forms of metal oxide nanoparticles; study of the various characterizing techniques that help deduce the shape, size, and morphology of these nanoparticles; and applications of these nanoparticles in the field of biosensors. It presents voltammetry techniques such as cyclic, linear wave, wave pulse, and differential pulse voltammetry, throws light on the interactions of nanomaterials and biomolecules, and discusses microfluidic devices, which due to their unique capability of miniaturization fascinate many researchers. It is a practical and user-friendly textbook that introduces the various basic principles and practical information that will help undergraduate and advanced-level students and researchers understand the science behind nanoscale sensing.

Concepts of Biology

Concepts of Biology
Author : Samantha Fowler,Rebecca Roush,James Wise
Publisher : Unknown
Release Date : 2018-01-07
Category : Science
Total pages :618
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Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.