April 17, 2021

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Microscope Image Processing

Microscope Image Processing
Author : Qiang Wu,Fatima Merchant,Kenneth Castleman
Publisher : Elsevier
Release Date : 2010-07-27
Category : Computers
Total pages :576
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Digital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology. Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and display, object measurement and classification, autofocusing, and structured illumination. Key Features: Detailed descriptions of many leading-edge methods and algorithms In-depth analysis of the method and experimental results, taken from real-life examples Emphasis on computational and algorithmic aspects of microscope image processing Advanced material on geometric, morphological, and wavelet image processing, fluorescence, three-dimensional and time-lapse microscopy, microscope image enhancement, MultiSpectral imaging, and image data management This book is of interest to all scientists, engineers, clinicians, post-graduate fellows, and graduate students working in the fields of biology, medicine, chemistry, pharmacology, and other related fields. Anyone who uses microscopes in their work and needs to understand the methodologies and capabilities of the latest digital image processing techniques will find this book invaluable. Presents a unique practical perspective of state-of-the-art microcope image processing and the development of specialized algorithms Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Microscope Image Processing

Microscope Image Processing
Author : Fatima Merchant,Kenneth Castleman
Publisher : Academic Press
Release Date : 2021-09-15
Category : Computers
Total pages :575
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Microscope Image Processing, Second Edition, introduces the basic fundamentals of image formation in microscopy including the importance of image digitization and display, which are key to quality visualization. Image processing and analysis are discussed in detail to provide readers with the tools necessary to improve the visual quality of images, and to extract quantitative information. Basic techniques such as image enhancement, filtering, segmentation, object measurement, and pattern recognition cover concepts integral to image processing. In addition, chapters on specific modern microscopy techniques such as fluorescence imaging, multispectral imaging, three-dimensional imaging and time-lapse imaging, introduce these key areas with emphasis on the differences among the various techniques. The new edition discusses recent developments in microscopy such as light sheet microscopy, digital microscopy, whole slide imaging, and the use of deep learning techniques for image segmentation and analysis with big data image informatics and management. Microscope Image Processing, Second Edition, is suitable for engineers, scientists, clinicians, post-graduate fellows and graduate students working in bioengineering, biomedical engineering, biology, medicine, chemistry, pharmacology and related fields, who use microscopes in their work and would like to understand the methodologies and capabilities of the latest digital image processing techniques or desire to develop their own image processing algorithms and software for specific applications. Presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Computer Vision for Microscopy Image Analysis

Computer Vision for Microscopy Image Analysis
Author : Mei Chen
Publisher : Academic Press
Release Date : 2020-12-01
Category : Computers
Total pages :228
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Are you a computer scientist working on image analysis? Are you a biologist seeking tools to process the microscopy data from image-based experiments? Computer Vision for Microscopy Image Analysis provides a comprehensive and in-depth discussion of modern computer vision techniques, in particular deep learning, for microscopy image analysis that will advance your efforts. Progress in imaging techniques has enabled the acquisition of large volumes of microscopy data and made it possible to conduct large-scale, image-based experiments for biomedical discovery. The main challenge and bottleneck in such experiments is the conversion of "big visual data" into interpretable information. Visual analysis of large-scale microscopy data is a daunting task. Computer vision has the potential to automate this task. One key advantage is that computers perform analysis more reproducibly and less subjectively than human annotators. Moreover, high-throughput microscopy calls for effective and efficient techniques as there are not enough human resources to advance science by manual annotation. This book articulates the strong need for biologists and computer vision experts to collaborate to overcome the limits of human visual perception, and devotes a chapter each to the major steps in analyzing microscopy images, such as detection and segmentation, classification, tracking, and event detection. Discover how computer vision can automate and enhance the human assessment of microscopy images for discovery Grasp the state-of-the-art approaches, especially deep neural networks Learn where to obtain open-source datasets and software to jumpstart his or her own investigation

Computer-Assisted Microscopy

Computer-Assisted Microscopy
Author : John C. Russ
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
Category : Science
Total pages :470
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The use of computer-based image analysis systems for all kinds of images, but especially for microscope images, has become increasingly widespread in recent years, as computer power has increased and costs have dropped. Software to perform each of the various tasks described in this book exists now, and without doubt additional algorithms to accomplish these same things more efficiently, and to perform new kinds of image processing, feature discrimination and measurement, will continue to be developed. This is likely to be true particularly in the field of three-dimensional imaging, since new microscopy methods are beginning to be used which can produce such data. It is not the intent of this book to train programmers who will assemble their own computer systems and write their own programs. Most users require only the barest of knowledge about how to use the computer, but the greater their understanding of the various image analysis operations which are possible, their advantages and limitations, the greater the likelihood of success in their application. Likewise, the book assumes little in the way of a mathematical background, but the researcher with a secure knowledge of appropriate statistical tests will find it easier to put some of these methods into real use, and have confidence in the results, than one who has less background and experience. Supplementary texts and courses in statistics, microscopy, and specimen preparation are recommended as necessary.

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging
Author : Douglas B. Murphy,Michael W. Davidson
Publisher : John Wiley & Sons
Release Date : 2012-08-22
Category : Medical
Total pages :552
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Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Electron Microscopy and Analysis

Electron Microscopy and Analysis
Author : Peter J. Goodhew,John Humphreys
Publisher : CRC Press
Release Date : 2000-11-30
Category : Technology & Engineering
Total pages :254
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Electron Microscopy and Analysis deals with several sophisticated techniques for magnifying images of very small objects by large amounts - especially in a physical science context. It has been ten years since the last edition of Electron Microscopy and Analysis was published and there have been rapid changes in this field since then. The authors h

Computer Processing of Electron Microscope Images

Computer Processing of Electron Microscope Images
Author : P. W. Hawkes
Publisher : Springer
Release Date : 2011-12-27
Category : Science
Total pages :296
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Towards the end of the 1960s, a number of quite different circumstances combined to launch a period of intense activity in the digital processing of electron micro graphs. First, many years of work on correcting the resolution-limiting aberrations of electron microscope objectives had shown that these optical impediments to very high resolution could indeed be overcome, but only at the cost of immense exper imental difficulty; thanks largely to the theoretical work of K. -J. Hanszen and his colleagues and to the experimental work of F. Thon, the notions of transfer func tions were beginning to supplant or complement the concepts of geometrical optics in electron optical thinking; and finally, large fast computers, capable of manipu lating big image matrices in a reasonable time, were widely accessible. Thus the idea that recorded electron microscope images could be improved in some way or rendered more informative by subsequent computer processing gradually gained ground. At first, most effort was concentrated on three-dimensional reconstruction, particu larly of specimens with natural symmetry that could be exploited, and on linear operations on weakly scattering specimens (Chap. l). In 1973, however, R. W. Gerchberg and W. O. Saxton described an iterative algorithm that in principle yielded the phase and amplitude of the electron wave emerging from a strongly scattering speci men.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author : James Pawley
Publisher : Springer Science & Business Media
Release Date : 2013-04-17
Category : Science
Total pages :632
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This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Digital Image Processing

Digital Image Processing
Author : Castleman
Publisher : Pearson Education India
Release Date : 2007-09
Category :
Total pages :686
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Bioimage Data Analysis Workflows

Bioimage Data Analysis Workflows
Author : Kōta Miura,Nataša Sladoje
Publisher : Springer Nature
Release Date : 2020-01-01
Category : Electron microscopy
Total pages :170
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The Essential Guide to Image Processing

The Essential Guide to Image Processing
Author : Alan C. Bovik
Publisher : Academic Press
Release Date : 2009-07-08
Category : Computers
Total pages :672
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A complete introduction to the basic and intermediate concepts of image processing from the leading people in the field Up-to-date content, including statistical modeling of natural, anistropic diffusion, image quality and the latest developments in JPEG 2000 This comprehensive and state-of-the art approach to image processing gives engineers and students a thorough introduction, and includes full coverage of key applications: image watermarking, fingerprint recognition, face recognition and iris recognition and medical imaging. "This book combines basic image processing techniques with some of the most advanced procedures. Introductory chapters dedicated to general principles are presented alongside detailed application-orientated ones. As a result it is suitably adapted for different classes of readers, ranging from Master to PhD students and beyond." – Prof. Jean-Philippe Thiran, EPFL, Lausanne, Switzerland "Al Bovik’s compendium proceeds systematically from fundamentals to today’s research frontiers. Professor Bovik, himself a highly respected leader in the field, has invited an all-star team of contributors. Students, researchers, and practitioners of image processing alike should benefit from the Essential Guide." – Prof. Bernd Girod, Stanford University, USA "This book is informative, easy to read with plenty of examples, and allows great flexibility in tailoring a course on image processing or analysis." – Prof. Pamela Cosman, University of California, San Diego, USA A complete and modern introduction to the basic and intermediate concepts of image processing – edited and written by the leading people in the field An essential reference for all types of engineers working on image processing applications Up-to-date content, including statistical modelling of natural, anisotropic diffusion, image quality and the latest developments in JPEG 2000

Microscopy and Analysis

Microscopy and Analysis
Author : Stefan G. Stanciu
Publisher : BoD – Books on Demand
Release Date : 2016-09-21
Category : Technology & Engineering
Total pages :442
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Microscopes represent tools of the utmost importance for a wide range of disciplines. Without them, it would have been impossible to stand where we stand today in terms of understanding the structure and functions of organelles and cells, tissue composition and metabolism, or the causes behind various pathologies and their progression. Our knowledge on basic and advanced materials is also intimately intertwined to the realm of microscopy, and progress in key fields of micro- and nanotechnologies critically depends on high-resolution imaging systems. This volume includes a series of chapters that address highly significant scientific subjects from diverse areas of microscopy and analysis. Authoritative voices in their fields present in this volume their work or review recent trends, concepts, and applications, in a manner that is accessible to a broad readership audience from both within and outside their specialist area.

The Image Processing Handbook

The Image Processing Handbook
Author : John C. Russ
Publisher : CRC Press
Release Date : 2016-04-19
Category : Technology & Engineering
Total pages :885
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Whether obtained by microscopes, space probes, or the human eye, the same basic tools can be applied to acquire, process, and analyze the data contained in images. Ideal for self study, The Image Processing Handbook, Sixth Edition, first published in 1992, raises the bar once again as the gold-standard reference on this subject. Using extensive new illustrations and diagrams, it offers a logically organized exploration of the important relationship between 2D images and the 3D structures they reveal. Provides Hundreds of Visual Examples in FULL COLOR! The author focuses on helping readers visualize and compare processing and measurement operations and how they are typically combined in fields ranging from microscopy and astronomy to real-world scientific, industrial, and forensic applications. Presenting methods in the order in which they would be applied in a typical workflow—from acquisition to interpretation—this book compares a wide range of algorithms used to: Improve the appearance, printing, and transmission of an image Prepare images for measurement of the features and structures they reveal Isolate objects and structures, and measure their size, shape, color, and position Correct defects and deal with limitations in images Enhance visual content and interpretation of details This handbook avoids dense mathematics, instead using new practical examples that better convey essential principles of image processing. This approach is more useful to develop readers’ grasp of how and why to apply processing techniques and ultimately process the mathematical foundations behind them. Much more than just an arbitrary collection of algorithms, this is the rare book that goes beyond mere image improvement, presenting a wide range of powerful example images that illustrate techniques involved in color processing and enhancement. Applying his 50-year experience as a scientist, educator, and industrial consultant, John Russ offers the benefit of his image processing expertise for fields ranging from astronomy and biomedical research to food science and forensics. His valuable insights and guidance continue to make this handbook a must-have reference.

Video Microscopy

Video Microscopy
Author : Shinya Inoue
Publisher : Springer Science & Business Media
Release Date : 2013-11-11
Category : Medical
Total pages :584
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Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

The Image Processing Handbook, Fifth Edition

The Image Processing Handbook, Fifth Edition
Author : John C. Russ
Publisher : CRC Press
Release Date : 2006-12-19
Category : Technology & Engineering
Total pages :832
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Now in its fifth edition, John C. Russ’s monumental image processing reference is an even more complete, modern, and hands-on tool than ever before. The Image Processing Handbook, Fifth Edition is fully updated and expanded to reflect the latest developments in the field. Written by an expert with unequalled experience and authority, it offers clear guidance on how to create, select, and use the most appropriate algorithms for a specific application. What’s new in the Fifth Edition? · A new chapter on the human visual process that explains which visual cues elicit a response from the viewer · Description of the latest hardware and software for image acquisition and printing, reflecting the proliferation of the digital camera · New material on multichannel images, including a major section on principal components analysis · Expanded sections on deconvolution, extended dynamic range images, and image enlargement and interpolation · More than 600 new and revised figures and illustrations for a total of more than 2000 illustrations · 20% more references to the most up-to-date literature Written in a relaxed and reader-friendly style, The Image Processing Handbook, Fifth Edition guides you through the myriad tools available for image processing and helps you understand how to select and apply each one.