November 30, 2020

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Understanding PCR

Understanding PCR
Author : Sarah Maddocks,Rowena Jenkins
Publisher : Academic Press
Release Date : 2016-10-27
Category : Science
Total pages :98
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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

PCR

PCR
Author : MCPHERSON.,Mike McPherson,S. G. Møller
Publisher : Garland Science
Release Date : 2000
Category : Juvenile Nonfiction
Total pages :276
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A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.

PCR

PCR
Author : Mike McPherson,Simon Møller
Publisher : Garland Science
Release Date : 2007-01-25
Category : Science
Total pages :292
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A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.

Understanding Viruses

Understanding Viruses
Author : Teri Shors
Publisher : Jones & Bartlett Publishers
Release Date : 2011-11-08
Category : Science
Total pages :736
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The ideal text for undergraduate students majoring in biology, microbiology, medical technology, or pre-med, the Second Edition of Understanding Viruses provides a balanced approach to this fascinating discipline, combining the molecular, clinical, and historical aspects of virology. Updated throughout to keep pace with this fast-paced field, the text provides a strong, comprehensive introduction to human viral diseases. New material on molecular virology as well as new virus families presented coupled with chapters on viral diseases of animals; the history of clinical trials, gene therapy, and xenotranplantation; prions and viroids; plant viruses; and bacteriophages add to the scope of the text. Chapters discussing specific viral diseases weave in an epidemiological and global perspective and include treatment and prevention information. Contemporary case studies, Refresher Boxes, and Virus Files engage students in the learning process. With a wealth of student and instructor support tools, Understanding Viruses is an accessible, exciting, and engaging text for your virology course.

PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology
Author : John P. Vanden Heuvel
Publisher : CRC Press
Release Date : 2019-05-07
Category : Medical
Total pages :240
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Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Principles and Technical Aspects of PCR Amplification

Principles and Technical Aspects of PCR Amplification
Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
Publisher : Springer Science & Business Media
Release Date : 2008-03-14
Category : Science
Total pages :330
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Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Polymerase Chain Reaction

Polymerase Chain Reaction
Author : Patricia Hernandez-Rodriguez
Publisher : BoD – Books on Demand
Release Date : 2012-05-30
Category : Science
Total pages :580
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This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.

The PCR Revolution

The PCR Revolution
Author : Stephen A. Bustin
Publisher : Cambridge University Press
Release Date : 2010
Category : Science
Total pages :307
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Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Ancient DNA Typing

Ancient DNA Typing
Author : Susanne Hummel
Publisher : Springer Science & Business Media
Release Date : 2003
Category : Law
Total pages :298
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This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.

Data Broadcasting: Understanding the ATSC Data Broadcast Standard

Data Broadcasting: Understanding the ATSC Data Broadcast Standard
Author : Richard S. Chernock,Regis J. Crinon,Michael A. Dolan,John R. Mick
Publisher : McGraw Hill Professional
Release Date : 2001-05-07
Category : Technology & Engineering
Total pages :621
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A ROADMAP FOR IMPLEMENTING THE ATSC DATA BROADCAST STANDARD The ATSC Data Broadcast Standard provides the means to transform digital TV signals from plain vanilla audio/video to 57-flavor broadband digital data delivery, opening a universe of device and service possibilities. Authored by four of the standard’s lead writers -- Richard Chernock, Regis Crinon, Michael Dolan, and John R. Mick, Jr. -- Data Broadcasting is the first guide to the new ATSC standard. These experts help you: *Understand the concepts behind standard provisions for carrying any type of data over MPEG-2 data transport mechanisms *Implement the standard in real-world service creation and rollouts *Enable a new generation of set-top boxes, hand-held devices, and PC add-in cards for handling both data and streaming video *Configure stand-alone data injection and data injection associated with video and audio programs *Define the optimal transmittal method for any type of data *Resolve system architecture, receiver-reference design, data categorization, and encapsulation questions *Solve announcement, and discovery and binding mechanisms problems *Avoid implementation pitfalls with encapsulation examples, illustrated layering, and buffer models

PCR Methods in Foods

PCR Methods in Foods
Author : John Maurer
Publisher : Springer Science & Business Media
Release Date : 2006-11-22
Category : Technology & Engineering
Total pages :148
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This book will introduce non-molecular biologists to diagnostic PCR-based te- nologies for the detection of pathogens in foods. By the conclusion of this book, the reader should be able to: 1) understand the principles behind PCR including real-time; 2) know the basics involved in the design, optimization, and imp- mentation of PCR in food microbiology lab setting; 3) interpret results; 4) know limitations and strengths of PCR; and 5) understand the basic principles behind a new fledgling technology, microarrays and its potential applications in food microbiology. This book will provide readers with the latest information on PCR and microarray based tests and their application towards the detection of bacterial, protozoal and viral pathogens in foods. Figures, charts, and tables will be used, where appropriate, to help illustrate concepts or provide the reader with useful information or resources as an important starting point in bringing molecular diagnostics into the food microbiology lab. This book is not designed to be a “cookbook”PCR manual with recipes and step-by-step instructions but rather serve as a primer or resource book for students, faculty, and other professionals interested in molecular biology and its integration into food safety. v Table of Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Chapter 1. PCR Basics Amanda Fairchild, M. S. , Margie D. Lee DVM, Ph. D. , and John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. The Mythology of PCR: A Warning to the Wise John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Chapter 3.

Understanding Bacteria

Understanding Bacteria
Author : S. Srivastava
Publisher : Springer Science & Business Media
Release Date : 2003-11-30
Category : Science
Total pages :469
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The discipline of microbiology that deals with an amazingly diverse group of simple organisms, such as viruses, archaea, bacteria, algae, fungi, and protozoa, is an exciting field of Science. Starting as a purely descriptive field, it has transformed into a truly experimental and interdisciplinary science inspiring a number of investigators to generate th a wealth of information on the entire gamut of microbiology. The later part of 20 century has been a golden era with molecular information coming in to unravel interesting insights ofthe microbial world. Ever since they were brought to light through a pair of ground glasses by the Dutchman, Antony van Leeuwenhoek, in later half of 17th century, they have been studied most extensively throughout the next three centuries, and are still revealing new facets of life and its functions. The interest in them, therefore, continues even in the 21 st century. Though they are simple, they provide a wealth of information on cell biology, physiology, biochemistry, ecology, and genetics and biotechnology. They, thus, constitute a model system to study a whole variety of subjects. All this provided the necessary impetus to write several valuable books on the subject of microbiology. While teaching a course of Microbial Genetics for the last 35 years at Delhi University, we strongly felt the need for authentic compiled data that could give exhaustive background information on each of the member groups that constitute the microbial world.

Understanding Genes and GMOs

Understanding Genes and GMOs
Author : Colin J. Sanderson
Publisher : World Scientific
Release Date : 2007
Category : Science
Total pages :345
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The rapid progress in genomics and related technologies has increased interest in genetically modified organisms (GMOs). This book equips you with information about what genes are, how they work, and how they can be modified and used in biotechnology. On environmental genetics, it also considers the risks of releasing agricultural GM plants.

Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers
Author : Morteza Jalali,Francesca Yvonne Louise Saldanha,Mehdi Jalali
Publisher : Academic Press
Release Date : 2017-03-31
Category : Science
Total pages :382
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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Understanding the Role of Neuronal MeCP2 in Rett Syndrome Using Genomic and Epigenomic Approaches

Understanding the Role of Neuronal MeCP2 in Rett Syndrome Using Genomic and Epigenomic Approaches
Author : Sailaja Peddada
Publisher : Unknown
Release Date : 2007
Category :
Total pages :296
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